Characterization of Cucurbita maxima Phloem Serpin-1 (CmPS-1)

Rapid affinity-purification and physicochemical characterization of pumpkin (Cucurbita maxima) phloem exudate lectin.

The chito-oligosaccharide-specific lectin from pumpkin (Cucurbita maxima) phloem exudate has been purified to homogeneity by affinity chromatography on chitin. After SDS/PAGE in the presence of 2-mercaptoethanol, the pumpkin phloem lectin yielded a single band corresponding to a molecular mass of 23.7 kDa, whereas ESI-MS (electrospray ionization MS) gave the molecular masses of the subunit as 2...

Rapid affinity-purification and physico- chemical characterization of pumpkin (Cucurbita maxima) phloem exudate lectin

Remote-controlled stop of phloem mass flow by biphasic occlusion in Cucurbita maxima

The relationships between damage-induced electropotential waves (EPWs), sieve tube occlusion, and stop of mass flow were investigated in intact Cucurbita maxima plants. After burning leaf tips, EPWs propagating along the phloem of the main vein were recorded by extra- and intracellular microelectrodes. The respective EPW profiles (a steep hyperpolarization/depolarization peak followed by a prol...

Cucurbit phloem serpins are graft-transmissible and appear to be resistant to turnover in the sieve element-companion cell complex.

Serpins are unique inhibitors of serine proteases that are located in various plant tissues and organs. An orthologue of the pumpkin (Cucurbita maxima) phloem serpin CmPS-1 was amplified from cucumber (Cucumis sativus) RNA by RT-PCR, cloned, and designated as CsPS-1 (GenBank accession no. AJ866989). Alternative amino acid sequences in the reactive centre loop suggest distinct inhibitory specifi...

Overview on Cucurbita Maxima Seed

Pumpkin ( cucurbita maxima ) belongs to cucurbitaceae which include pumpkin, gourds, melons and squashes. They are characterized by their seed oil. Pumpkin plant has been grown since the earliest history of mankind 1 . ( Brucher – 1989 ). Pumpkin are grown around the world for variety of agricultural purposes, the seeds show anti helmintic, taenicide diuretic, cardio tonic, anti-inflammatory, a...